It is highly efficient (thus reducing RA time), robust for both single cell and single nucleus samples), with increased replicability (easier interface provides reduced variability between experiments), wide applicability across tissue types (has successfully produced data across a spectrum of tissues including nervous system, adipose, pancreas, liver, endothelial cells, muscle), and flexible (stand-alone single-nucleus ATAC-seq as well as simultaneous single cell multi-ome ATAC + Gene Expression kits are now available), with excellent company support. ġ0x Genomics Chromium Controller device: The Chromium Controller uses microfluidics to perform single cell partitioning and barcoding in minutes. Efficiency is enhanced via reaction miniaturization as well as reduced dead volumes and tip consumption. It uses a diaphragm-based chip design and is thus liquid class compatible, dispensing aqueous and organic solutions of varying viscosities down to 100nL at CV < 3%. It provides highly precise analytical evaluation of RNA and DNA samples for objective assessment of sizing, quantitation, integrity and purity from RNA input, cDNA synthesis, as well as sequencing library quality and sizing using minimal sample volume.įormulatrix MANTIS Liquid Handler: The MANTIS automated liquid handler increases the reproducibility, throughput and efficiency of our RNA-seq workflows. We also provide customized analysis of NGS data, specializing in integration between genetic, transcriptional, and epigenetic datasets.Īgilent 2100 Bioanalyzer: The Agilent Bioanalyzer is an established automated electrophoresis tool for the sample quality control of biomolecules. For data generated in-house, all data is processed through in-house pipelines that adapt widely-used analysis software (e.g., edgeR, Seurat, CellRanger, CellBender) to provide best practices, high quality, interfaceable data back to the user, including standard PCA, mean-difference, and volcano plots, pathway analysis, and formatted data that can be easily loaded onto platforms such as the Broad Single Cell Portal or Morpheus matrix visualization and analysis.
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